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pMC_mNG2(11)_BSDminus_F0

Plasmid

#184162

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(11)
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 WT MYOC eGLuc2 FLAG

Plasmid

#192820

Purpose

Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

Depositor

John Hulleman

Article

Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

Insert

myocilin (MYOC Human)

Tags

FLAG and enhanced Gaussia luciferase (M43I/M110I)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTS2337_Tier3(SB)-TagBFP2_Puro

Plasmid

#169635

Purpose

Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven mTagBFP2 and PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAtCas9-D10A_2

Plasmid

#91160

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Smad5 CDS

Plasmid

#61800

Purpose

pcDNA3.1(+) with full length mouse Smad5 CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Smad5 CDS (Smad5 Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pMOD_A0801

Plasmid

#91022

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg1

Plasmid

#87189

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 1

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #1 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGP8GF-AtTIR1

Plasmid

#188427

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor TIR1

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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EYFP-Clathrin-19

Plasmid

#56584

Purpose

Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin (CLTB Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMOD_A1610

Plasmid

#91038

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1

Plasmid

#188963

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: atcggtcgcattgttttccactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTC365

Plasmid

#91214

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-RbE2Fmut

Plasmid

#32391

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated E2F binding site: TTTCCCGC --> TTTACATC

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pTaCas9_6

Plasmid

#91169

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSBL_DK193

Plasmid

#156424

Purpose

Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein (Q57H)

Tags

sfGFP-His

Expression

Insect

Mutation

Q57H

Promoter

Polyhedrin

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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