We narrowed to 10,528 results for: SEC

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pcDNA3-Flag-NSF/S647A

Plasmid

#74938

Purpose

Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

S647A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pHalo2X-mMYO7A-RK/AA

Plasmid

#251426

Purpose

Mammalian expression vector for expressing a HaloTag-fused mouse MYO7A mutant that destabilizes the autoinhibitory conformation.

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (RK/AA) (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

Two missense mutations in the RGSK motif of the s…

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Article

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK ΔCRD-Fc

Plasmid

#246706

Purpose

Expression vector for secreted human RECK lacking the CRD, fused to mouse IgG2a Fc

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

Δaa 343-476, ΔGPI anchor signal

Promoter

CMV

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK ΔCK-Fc

Plasmid

#246705

Purpose

Expression vector for secreted human RECK lacking CC domains 1-5, fused to mouse IgG2a Fc

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

Δaa 37-338, ΔGPI anchor signal

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK-Fc

Plasmid

#246704

Purpose

Expression vector for a secreted human RECK-mouse IgG2a Fc fusion protein

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

ΔGPI anchor signal

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-9-sGpr124

Plasmid

#246710

Purpose

Expression vector for secreted mouse Gpr124 ECD, N-terminal 3xFLAG tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

Gpr124 (Adgra2 Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sRECK-His

Plasmid

#246688

Purpose

Expression vector for secreted human RECK, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

RECK (RECK Human)

Tags

6xHis

Expression

Mammalian

Mutation

ΔGPI anchor signal

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Igκ SP-sGPR124-Fc

Plasmid

#246684

Purpose

Expression vector for a secreted human GPR124 ECD-mouse IgG2a Fc fusion protein

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

mouse IgG2a Fc

Expression

Mammalian

Mutation

ECD

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP6 57-98

Plasmid

#242420

Purpose

Expression of the second alpha helix of CHMP6 (residues 57-98), N-terminally tagged with sfGFP

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP6 (CHMP6 Human)

Tags

sfGFP (superfolder GFP)

Expression

Mammalian

Mutation

Residues 57-98

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-UL36USP (C40)

Plasmid

#235548

Purpose

To express GFP-FLAG-UL36USP (C40) - region 25-520 of UL36

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

UL36USP (C40)

Use

Lentiviral

Tags

EGFP-FLAG

Mutation

N-terminal part of UL36 starting at the second AT…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-C

Plasmid

#228570

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_PST-N

Plasmid

#228571

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Pancreastatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-C

Plasmid

#228572

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-N

Plasmid

#228573

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

Plasmid

#223764

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

Plasmid

#223765

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_GST-TEV-CK2alpha_CK2beta

Plasmid

#223740

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

CK2-alpha, CK2-beta (CSNK2A1 Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_GST-TEV-CK2alpha'_CK2beta

Plasmid

#223741

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

CK2-alphaprime_CK2-beta (CSNK2A2 Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pEmal10H-MBP-EWSR1-449-513

Plasmid

#199439

Purpose

Expresses RG2 (the second RG-rich region, 449-513) of EWS

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Tags

His10 and MBP

Expression

Bacterial

Mutation

deleted amino acids 1-448, 514-656

Promoter

T7

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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