We narrowed to 17,698 results for: puromycin
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Plasmid#142277PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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TFORF0766
Plasmid#142204PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2570
Plasmid#142122PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0116
Plasmid#141523PurposeLentiviral vector for overexpressing the FOXM1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0059
Plasmid#141500PurposeLentiviral vector for overexpressing the CTBP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-KO-Puro-TP53
Plasmid#227319PurposeDonor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)DepositorInsertTP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-CLTC
Plasmid#227314PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-mTagBFP2-Sec61B
Plasmid#228136PurposeExpresses mTagBFP2-Sec61B in mammalian cellsDepositorAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_CMV_cMyc-VSVG
Plasmid#212644PurposeLentivirus vector to express cMyc-VSVGDepositorInsertVSVG
UseLentiviralTagscMyc tagExpressionMammalianPromoterCMVAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABEmax-Puro V2
Plasmid#226955PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-BTC-ScNeo
Plasmid#209898PurposeTo monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-Necl5-ScNeo
Plasmid#209901PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-NRG1-ScNeo
Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNRG1-ScNeo (NRG1 Human)
TagsmNeonGreen and mScarletExpressionMammalianAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-GFP-TurboID-HA-rtTA
Plasmid#226996PurposeIntegrative plasmid to express GFP-TurboID in mammalian cellsDepositorAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11-MMEJ
Plasmid#227333PurposeMMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-TUBA1B
Plasmid#227321PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
(attB) tdMCP-tagged BRaf
Plasmid#226165PurposeUsed to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.DepositorInsert3xFlag-tdMCP-BRaf (BRAF Human)
UseBxb1 attb, no promoterTags3xFlag-tdMCP and IRES-mCherry-P2A-PuroRExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-H2BC21
Plasmid#227589PurposeLentiviral plasmid expressing human H2BC21 proteinDepositorAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mECFP
Plasmid#223954PurposeGateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mECFPAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only