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pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F3

Plasmid

#236448

Purpose

insect expression of E2F3 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3 (E2f3 Mouse)

Expression

Insect

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-HOIL-1L NZF (195-252)

Plasmid

#235650

Purpose

Bacterial expression of human HOIL-1L NZF (195-252)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

HOIL-1L NZF (RBCK1 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 195-252 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-LEO1

Plasmid

#231727

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

LEO1 (LEO1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-PAF1

Plasmid

#231728

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

PAF1 (PAF1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Cyclin T1

Plasmid

#231720

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CCNT1 (CCNT1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0216 CMVTO-hIL6Ra_M4-rens-hIL6-rens-IL6Rb

Plasmid

#231194

Purpose

Multi-cageIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6Ra_M4-rens-hIL6-rens-IL6Rb (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

Plasmid

#231208

Purpose

Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)

Use

Lentiviral

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0251 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens

Plasmid

#231216

Purpose

myc-CleaveIL-10

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

c-myc-mono-hIL10 (R5A11M)-rens (IL10 Human)

Expression

Mammalian

Mutation

N36I, N110I, K117N, F129L

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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