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pMEF2c-AHF-Dre-nls (JDW 35)

Plasmid

#242585

Purpose

The Mef2c anterior heart field promoter driving expression of Dre recombinase.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE-nls

Expression

Mammalian

Promoter

mouse Mef2c

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-mKate (JDW 494)

Plasmid

#242575

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

Plasmid

#242587

Purpose

A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nlsKateV5, MbBPF-FLAG, FRTNeoFRT

Expression

Mammalian

Promoter

CAGGS

Available since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-DreERT2 (JDW 196)

Plasmid

#242586

Purpose

The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Expression

Mammalian

Promoter

mouse Mef2c

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

Plasmid

#229841

Purpose

A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-H2B-mCerulean-2xStop-loxP

Use

Cre/Lox; Tol2 based expression vector

Available since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pXMLC2-DEST (JDW 7)

Plasmid

#229833

Purpose

A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway Cloning

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-BFP (JDW 491)

Plasmid

#229834

Purpose

A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

Plasmid

#229837

Purpose

A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB444

Plasmid

#74830

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Publication

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS SK mCherryROSAegfp

Plasmid

#54322

Purpose

Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

Depositor

Silvestro Conticello

Publication

Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

Insert

mCherry and EGFP

Use

CRISPR, Mouse Targeting, and TALEN

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_mCherry

Plasmid

#221470

Purpose

Gateway cloning of mCherry overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

mCherry

Use

Gateway Cloning

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pC031 - RcrCas13a from Rhodobacter capsulatus R121

Plasmid

#91921

Purpose

Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcrCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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