We narrowed to 10,590 results for: Kars;

1. pGEX-4T-1 dATG1 (1188)

   Plasmid

   #66918

   Purpose

   bacterial expression of dATG1

   Depositor

   Michael Karin, Jun Hee Lee

   Article

   Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

   Insert

   ATG1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBsaFa

   Plasmid

   #85104

   Purpose

   FAD domain of P450 BM3 variant (C773S/C810S)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   FADdomain of P450 BM3
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   C773S/C810S

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBsaPro5X

   Plasmid

   #85100

   Purpose

   Putidaredoxin fused to Pro5

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHPro51

   Plasmid

   #85097

   Purpose

   1st Pro5

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Gly-Gly-Gly-Gly-Ser-Pro-Pro-Pro-Pro-Pro
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUG35-YDL183c-GFP

   Plasmid

   #70161

   Purpose

   yeast expression of YDL183c with GFP fusion

   Depositor

   Karin Nowikovsky

   Article

   Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

   Insert

   YDL183c
   

   Use

   Tags

   yEGFP

   Expression

   Yeast

   Mutation

   Promoter

   MET17

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pUC19_extendedTTE1564

   Plasmid

   #60999

   Purpose

   T7-transcription template for Tte mRNA used in SiM-KARTS

   Depositor

   Nils Walter

   Article

   Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

   Insert

   TTE1564 transcript
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSG5-p85α_wt

   Plasmid

   #39497

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   p85 (PIK3R1 Bovine)
   

   Use

   Eukaryotic

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pGEX2T E7 del PTLHE

   Plasmid

   #13637

   Depositor

   Karl Munger

   Article

   Wu et al J Virol. 1993 Apr . 67(4):2402-7.

   Insert

   HPV 16 E7 del PTLHE (E7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   del aa 6-10

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pSANG10-3F-BG4

   Plasmid

   #55756

   Purpose

   Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

   Depositor

   Shankar Balasubramanian

   Article

   Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

   Insert

   BG4
   

   Use

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin of AcMNPV

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTXB1-Tn5

    Plasmid

    #60240

    Purpose

    IPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Rickard Sandberg

    Article

    Picelli et al Genome Res. 2014 Jul 30. pii: gr.177881.

    Insert

    tnpA from pBam1
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7 promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIP

    Plasmid

    #79009

    Purpose

    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hNox5

    Plasmid

    #69354

    Purpose

    expresses human Nox5 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

    Insert

    hNox5 (NOX5 Human)
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-pLKO-FOXM1-shRNA-81

    Plasmid

    #89497

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tet-pLKO-FOXM1-shRNA-#2

    Plasmid

    #89499

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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