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pCfB2792

Plasmid

#63653

Purpose

pCfB2792 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSCKtheoRaj12m

Plasmid

#69943

Purpose

pSTC2,TheoHHAzRaj12mut,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Publication

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

pSCKtheoRaj12m

Use

Synthetic Biology; Psc101 origin of replication

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTHSSd_40

Plasmid

#59963

Purpose

High core fragment expression without SynZIP

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment lacking SynZIP

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSA39

Plasmid

#35110

Depositor

John McCusker

Publication

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5MX4

Use

Yeast replicating

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSA53

Plasmid

#35115

Depositor

John McCusker

Publication

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

callys5 flanked by loxp

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pPA101

Plasmid

#17256

Depositor

Nancy Craig

Publication

Gamas et al Nucleic Acids Res. 1992 May 25. 20(10):2

Insert

TnsC (tnsC E. coli Tn7)

Expression

Bacterial

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

Plasmid

#239028

Purpose

Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

Depositor

Josh Huang

Publication

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Satb2 (Satb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG1B-eIF3hdm

Plasmid

#240038

Purpose

Human eIF3h, eIF3d and eIF3m subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Publication

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3h, d, m

Tags

eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLR06_pGuide_B2M_sgRNA2

Plasmid

#131091

Purpose

sgRNA for SpCas9 mediated B2M KO

Depositor

Xiaojun Lian

Insert

B2M KO sgRNA 2 (B2M Human)

Use

CRISPR

Expression

Mammalian

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Publication

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAT

Plasmid

#208339

Purpose

ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

Depositor

Philippe Soriano

Publication

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SAT sensor

Use

Mouse Targeting

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Publication

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-Gamillus

Plasmid

#197920

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pAto-mCherry

Plasmid

#192858

Purpose

pMUT2 based vector for sensing acetoacetate in E. coli Nissle 1917

Depositor

Morten Sommer

Publication

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Acetoacetate inducible promoter

Expression

Bacterial

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG2_mic

Plasmid

#184916

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ipUSEPR-sg-Hs-BCL2L1-A1

Plasmid

#188692

Purpose

sgRNA

Depositor

Lewis Cantley

Insert

sgBCL2L1 (BCL2L1 Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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