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We narrowed to 62,227 results for: cloning

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Showing: 9361 - 9380 of 62227 results

  1. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  2. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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  3. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  4. FB003

    Plasmid
    #119677
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119677-plasmid-map-sequence-id-238439

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  5. FB010

    Plasmid
    #119708
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119708-plasmid-map-sequence-id-236964

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  6. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

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  7. FB002

    Plasmid
    #119676
    Purpose
    Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator tub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119676-plasmid-map-sequence-id-237306

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  8. FB008

    Plasmid
    #119706
    Purpose
    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119706-plasmid-map-sequence-id-237146

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  9. FB012

    Plasmid
    #119709
    Purpose
    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119709-plasmid-map-sequence-id-237149

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  10. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  11. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  13. pBS_GGSG_mAID_HA_BactBsd

    Plasmid
    #235504
    Purpose
    single mAID_3xHA cloning vector with Roxed Bact-Bsd
    Depositor
    Brian Hendrich
    Insert
    mini-Auxin Inducible Degron
    Use
    Mouse Targeting
    Tags
    mAID, HA
    Mutation
    None
    Promoter
    beta actin
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235504-plasmid-map-sequence-id-474046

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  14. mEmerald-TPX2-M1-C-18

    Plasmid
    #62764
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2M1 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K313A, K314A, R315A Alanine mutagenesis
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. mEmerald-TPX2T4-C-18

    Plasmid
    #62766
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2T4 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Truncated TPX2 M2 AA (713-747)
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. mEmerald-TPX2-M2-C-18

    Plasmid
    #62765
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2M2 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K158A, K159A, K313A, K314A, R315A Alanine mutagen…
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pTol2-Crestin-zFUCCI-IRES-EGFP-caax (JDW 1516)

    Plasmid
    #242601
    Purpose
    A Tol2 expression vector containing the Crestin enhancer driving FUCCI to label cell cycle in neural crest cells followed by an IRES-EGFP-caax to label all neural crest cells green.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mCerulean-zGEM-P2A-mCherry-zCdt1
    Use
    Zebrafish expression
    Promoter
    Crestin enhancer
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    242601-plasmid-map-sequence-id-505637

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Showing: 9361 - 9380 of 62227 results