We narrowed to 8,418 results for: construct

1. pMBD1.1.0-gDNA

   Plasmid

   #132444

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   MBD1 (MBD1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK523

   Plasmid

   #72442

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)

   Depositor

   T. J. Kappock

   Insert

   hypothetical protein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene with ~40 silent substitutions

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK138

   Plasmid

   #73314

   Purpose

   Pas2p peroxisomal membrane targeting sequence

   Depositor

   T. J. Kappock

   Insert

   Pas2p (peroxisomal membrane targeting region)
   

   Use

   Tags

   His6 and c-myc

   Expression

   Yeast

   Mutation

   encodes residues 1-42

   Promoter

   AOX1

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK126

   Plasmid

   #73337

   Purpose

   Encodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)

   Depositor

   T. J. Kappock

   Insert

   electron transfer flavoprotein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   encodes residues 328-685

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK108

   Plasmid

   #73312

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK322

   Plasmid

   #73494

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   n/a

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK125

   Plasmid

   #73336

   Purpose

   Encodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)

   Depositor

   T. J. Kappock

   Insert

   electron transfer flavoprotein
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   encodes residues 1-327

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK553

   Plasmid

   #72452

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   synthetic gene with ~40 silent substitutions

   Promoter

   T7

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK636

   Plasmid

   #72466

   Purpose

   Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)

   Depositor

   T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Tags

    Expression

    Mutation

    Promoter

    C. glabrata MET3

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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