-
Plasmid#60885PurposeFPX biosensor for caspase-1DepositorInsertsingle polypeptide FPX biosensor for caspase-1
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for caspase-8
Plasmid#60884PurposeFPX biosensor for caspase-8DepositorInsertsingle polypeptide FPX biosensor for caspase-8
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
single polypeptide FPX biosensor for both caspase-3 and -8
Plasmid#60886PurposeFPX biosensorDepositorInsertsingle polypeptide FPX biosensor for caspase-3 and 8
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-CAAX
Plasmid#208683PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cellsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP-CAAX
Plasmid#208684PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neuronsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP-CAAX
Plasmid#208685PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent mannerDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-8
Plasmid#224781PurposeLanding Pad donor vector for AAVS1 site integration in human and VeroE6 cellsDepositorInsertspuromycin N-acetyltransferase
enhanced Green Fluorescence Protein
Red Fluorescence Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
Plasmid#74101Purposevector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015DepositorInsertsGreen Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mTq2-antiFRET-P2A2-linker_noSacI
Plasmid#191472PurposeNegative control for ratiometric comparison between red fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGExpressionMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCri-8a-Strep
Plasmid#61340PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-4a-Strep
Plasmid#61338PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag, Strep-tag, and TRX-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-1a-Strep
Plasmid#61337PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tag and MBP-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_3X Flag
Plasmid#194161PurposeExpresses eGFP fluorescent protein with a 3X flag tagDepositorInsertGFP with 3X Flag
UseTags3x FlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tubulin-hfYFP
Plasmid#186515PurposeLocalization of hyperfolder YFP fluorescent protein to tubulinDepositorInsertHyperfolder YFP
UseTagsTubulinExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only