We narrowed to 1,134 results for: fret

1. Vh-Clover-CC-S7-mRuby2-Vt

   Plasmid

   #233229

   Purpose

   Vinculin FRET tension sensor with coiled-coil S7 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S7-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Vh-Clover-CC-Mad-mRuby2-Vt

   Plasmid

   #233232

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-Mad-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Vinculin-Clover-CC-S0-mRuby2

   Plasmid

   #233233

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S0-mRuby2
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBbsr2-TSen

   Plasmid

   #209916

   Purpose

   A FRET biosensor for ADAM17 activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TSen
   

   Tags

   ECFP and YPet

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S::SP-mCherry-GFP-HDEL

   Plasmid

   #159097

   Purpose

   Positive control for FRET in plant ER / nuclear envelope.

   Depositor

   Joe McKenna

   Article

   McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

   Insert

   SP-mCherry-GFP-HDEL
   

   Tags

   mCherry, GFP

   Expression

   Plant

   Promoter

   35S

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDx_FusionRed-SYFP2

   Plasmid

   #189760

   Purpose

   Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   FusionRed-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDx_mScarlet-SYFP2

   Plasmid

   #189762

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-FKBP-E2

   Plasmid

   #175243

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

    Plasmid

    #138197

    Purpose

    FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

    Plasmid

    #138199

    Purpose

    FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

    Plasmid

    #138200

    Purpose

    FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains leucine-to-tryptophan substitution at re…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Human, Synthetic)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Tags

    T2A peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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