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pAAV-Ef1a-Con/Fon-BFP

Plasmid

#137129

Purpose

Intersectional viral expression of BFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-hSyn-NCreIntN-T2A-mTagBFP2

Plasmid

#160502

Purpose

Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19/PLIN2 GFP-P2A-BFP

Plasmid

#202195

Purpose

knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Insert

PLIN2 (PLIN2 Human)

Use

Cloning vector

Tags

GFP-P2A-BFP knock-in

Expression

Mutation

Promoter

lac

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-PIF-SOS2cat

Plasmid

#50850

Purpose

Optogenetic Ras activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Inserts

SOS2cat (SOS2 Human)
PIF6 1-100

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

residues 566–1049

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAF0690 Ef1a-Kp03_attB-BFP acceptor

Plasmid

#193473

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mTagBFP2-2A-FLAG-ntcGAS

Plasmid

#102603

Purpose

Lentivector to express Flag-tagged and shRNA-resistant cGAS and mTagBFP

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti Vp64-dCas9 T2A mTagBFP2

Plasmid

#171052

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Vp64-dCas9 T2A mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKIIa-moxBFP-IRES-Flpo

Plasmid

#194977

Purpose

AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

moxBFP, Flpo

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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hGFAP-NLS-HA-Dre-P2A-BFP

Plasmid

#51277

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Dre-P2A-BFP

Use

Tags

Expression

Mutation

Promoter

hGFAP

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

Plasmid

#176458

Purpose

Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

Depositor

Hui Yang

Article

Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

Insert

humanized S. pyogenes Cas9-XTEN-Ec73RT fusion

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk D319N

Plasmid

#102350

Purpose

Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

Depositor

Jared Toettcher

Article

Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

BFP

Expression

Mutation

Aspartic acid 319 was mutated to asparagine

Promoter

SFFV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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XClone-Cas9-Cronus-T2A-BFP

Plasmid

#141209

Purpose

Tunable and temporal expression control of Cas9

Depositor

Rory Johnson

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pscAAV-CBh-tagBFP2-WPRE3-enSV40pA

Plasmid

#194246

Purpose

Expresses tagBFP2

Depositor

Michael J Fox Foundation MJFF

Insert

tagBFP2

Use

AAV

Tags

Expression

Mutation

Promoter

CBh

Available since

May 8, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCCL-PGK-SPdCas9-BFP-DNMT3A

Plasmid

#66819

Purpose

dCas9 fused to BFP and the human DNMT3A catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A

Use

Tags

dSpCas9-BFP-DNMT3A

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

Plasmid

#178868

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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