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Plasmid#125244PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRGECO2
UseTagsExpressionMammalianMutationW44Y, D432APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGREAT2
Plasmid#170890PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT1
Plasmid#170889PurposeGoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr
Plasmid#216113PurposeExpresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertCAG_oROS-Gr
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRCaMP2
Plasmid#125250PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRCaMP2
UseTagsExpressionMammalianMutationD396APromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet-I3-NCwt_N1
Plasmid#189779PurposeConstruct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet-I3-NCwt
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CAG_3C-Twin-Strep
Plasmid#113901PurposeMammalian protein expressionDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCAGAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP3bright
Plasmid#74423PurposeExpress fluorescent calcium indicator GCaMP3bright in mammalian cellsDepositorInsertGCaMP3bright
UseTagsExpressionMammalianMutationD392APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRCaMP1
Plasmid#125249PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRCaMp1
UseTagsNES signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorTagsExpressionMutationPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-Pepper-RhoBAST
Plasmid#196343PurposeOverexpression of circular Pepper-RhoBASTDepositorInsertTornado-Pepper-RhoBAST
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-eNpHR-EYFP-WPRE
Plasmid#20946Purpose3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorInserthalorhodopsin
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGREAT26
Plasmid#170914PurposeGoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpEstradiol_InducibleAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-RapACR_T111C-EYFP
Plasmid#119195PurposeFast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spikingDepositorInsertAnion channelrhodopsin RapACR fast mutant T111C
UseLentiviralTagsEYFPExpressionMutationchanged threonine 111 to cysteinePromoterUbiquitinAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-APEX2m
Plasmid#176843PurposeExpression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-APEX2m
UseIntegratingTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRGECO1
Plasmid#125247PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only