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pGP-CMV-NES-jRCaMP1b

Plasmid

#63136

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1b

Use

Tags

nuclear export signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 LRP5

Plasmid

#115907

Purpose

Expresses human LRP5 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn A30P

Plasmid

#105729

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein A30P (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

A30P

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

Plasmid

#100848

Purpose

AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1 and AAV9

Insert

jRCaMP1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn E46K

Plasmid

#105730

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Use

Tags

Expression

Mammalian

Mutation

E46K

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdPCP stdGFP

Plasmid

#104099

Purpose

Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdPCP-stdGFP

Use

Lentiviral

Tags

N-terminal NLS, Two synonymous copies of GFP (C-t…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFr.WPRE.SV40

Plasmid

#98929

Purpose

AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgArid1a-sg3

Plasmid

#192169

Purpose

Lenti-sgArid1a-sg3

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

Plasmid

#78101

Purpose

Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Mutation

Promoter

pEF alpha

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

Plasmid

#123363

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

EGFP-NLS

Use

CRISPR, Lentiviral, and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaMPARI W391F V398L

Plasmid

#60787

Purpose

Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI W391F V398L

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pETDUET-GST AO7REwt

Plasmid

#213935

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

AO7 cloned into the first multiple cloning site o…

Promoter

T7 promoter-1

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorA(sp)-mNeonGreen

Plasmid

#205020

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorA-mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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