We narrowed to 15 results for: MB
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TypeBlog Post...proteolysis of the expressed proteins. hsdSB (rB- mB-): These strains have an inactivated native restriction... containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) General protein expression BL21...p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) pLysS (CamR) Expression of toxic...p15A origin of replication F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) pLysE (CamR) Expression of toxic...longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131 (DE3) General expression; not recommended...be required for expression F- ompT lon hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA General protein expression...with repetative sequences. F- ompT lon hsdSB (rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR) Expression...
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New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...expressing MDA-MB-231 cells (top, scalebar = 10µm) and TEM of GVs purified from MDA-MB-231 cell lysate...transfection. Fig: 2: Expressing mARGAna in MDA-MB-231 cells. (a) Plasmid diagram of mARGAna. (b) Schematic... scalebar = 0.5µm). (d) Ultrasound imaging of MDA-MB-231 tumors after 12-days of in situ expression. The... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1...storage of pBR322 plasmids. F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl... wash step. F- glnV44 recA13 mcrB mrr hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5 leu mtl-1... -
5 Great Apps for Lab Life
TypeBlog Post...The free subscription to Lab Archives allots for 25 MB of space per user, but, if you need more space, you... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...fast and can cut in as little as 2 hours. Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post... Windsor I, Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ... -
Lentiviral Vector Uses and Overview
TypeBlog Post...lentiviral vectors: progress and applications. Banasik MB, McCray PB Jr. Gene Ther. 2010 Feb;17(2):150-7. PubMed... -
QC Sequencing Technologies at Addgene
TypeBlog Post...provide longer read lengths of 10 kb to 100 kb (or 4 Mb in ultra-long read mode), enough to sequence an entire... -
CRISPR Plasmids - Tagging
TypeCollection...tagging in Drosophila cells 3.2 MB C terminal tagging in Drosophila cells 3.3 MB Seydoux C. elegans Tagging... -
Genetic Code Expansion
TypeCollection...M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS C6 C6 HaloTyrosine tRNA synthatase M. barkeri...Amino Acids Bacterial TAG Ryan Mehl 160378 pDule2-Mb haloTyrRS C6 C6 HaloTyrosine tRNA synthatase M. barkeri...PylRS M. alvus Bacterial Ryan Mehl 197576 pAcBac1-Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated...derivatives Mammalian TAG Ryan Mehl 197577 pAcBac1-Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated... -
CRISPR References and Information
TypeCollection...pAC148 , pAC149 ; Gateway destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; ...pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector... -
Molecular Biology Reference
TypeGuide... New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB Survival Invitrogen F- mcrA Delta(mrr-hsdRMS-mcrBC...gyrA462 endA Delta(sr1-recA) mcrB mrr hsdS20 (rB- mB-) supE44 ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5...-mcrBC) Stbl3 Invitrogen F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Mammalian U6 none Fn Cpf1 Kim pU6-Mb-crRNA 78959 Mammalian U6 none Mb Cpf1 Kim pCAG-SpCas9-GFP-U6-gRNA ... -
Immunology Research Plasmids and Resources
TypeCollection...CD79a molecule, immunoglobulin-associated alpha IGA, MB-1 CD79B CD79b molecule, immunoglobulin-associated... -
Neurodegeneration Plasmid Collection
TypeCollection...diffuse leukoencephalopathy Dong-Er Zhang 12422 pMCSF-R(mB)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy...