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pRS423-FjTAL(JBEI-15466)

Plasmid

#87936

Purpose

Expression in S.cerevisiae of FjTAL

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Insert

FjTAL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pRSETB

Plasmid

#67658

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 M337V

Plasmid

#27460

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Methionine 337 mutated to Valine (M337V)

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Use

Tags

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRS405 PEX14-VAC8-RFP

Plasmid

#166844

Purpose

Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, PEX14 Budding Yeast)

Use

Tags

PEX14 transmembrane domain and RFP

Expression

Yeast

Mutation

PEX14 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 SEC66-VAC8-RFP

Plasmid

#166843

Purpose

Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8, SEC66 Budding Yeast)

Use

Tags

RFP and SEC66 transmembrane domain

Expression

Yeast

Mutation

SEC66 transmembrane domain

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 Q331K

Plasmid

#27461

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRSET-QUEEN 7mu A81D

Plasmid

#129351

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81D

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu E83A

Plasmid

#129353

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu E83A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Sean Munro, Tim Levine

Article

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1-YFP

Plasmid

#18685

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen-StefinA

Plasmid

#182419

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRS2 (HIF2a-pRetro-Super)

Plasmid

#22100

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRS9 (HIF2a-pRetro-Super)

Plasmid

#22101

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-sctetR-FokI

Plasmid

#44756

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

sctetR-FokI (fokI Synthetic)

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1pr

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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