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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_LolT_C41S

Plasmid

#211789

Purpose

Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

Depositor

Yang Hai

Article

Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

Insert

lolt mutant

Tags

His tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

F13A

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hisA cdc25c S216A

Plasmid

#10968

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c S216A (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

S216A. Serine to Alanine substitution at aa216.

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro

Plasmid

#177201

Purpose

encoding a dominant negative form of CEBP under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

dnCEBP (A-C/EBP)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAW206

Plasmid

#113246

Purpose

E. coli expression vector for IHFa K15A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K15A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K15A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#113234

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->CCATC

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4929

Plasmid

#126751

Purpose

Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

ALD6

Expression

Yeast

Promoter

pTRX2_5xUAS

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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GST cdc25c C330S C377S

Plasmid

#10970

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S C377S (CDC25C Human)

Tags

GST

Expression

Bacterial

Mutation

C330S C377S. Cysteine to Serine substitution at …

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1hisA cdc25c C330S

Plasmid

#10966

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

C330S. Cysteine to Serine substitution at aa330.

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-6xHis-TrxF

Plasmid

#207454

Purpose

E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

Depositor

Joseph Jez, Sarah Stainbrook

Article

Stainbrook et al bioRxiv 2022.08.24.505184

Insert

Thioredoxin F

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

T7

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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A0A0H2LZ12

Plasmid

#163259

Purpose

Inducible expression of UniProt identifier A0A0H2LZ12

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0H2LZ12

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW207

Plasmid

#113247

Purpose

E. coli expression vector for IHFa K20A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K20A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K20A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW205

Plasmid

#113245

Purpose

E. coli expression vector for IHFa D14A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa D14A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

D14A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW217

Plasmid

#113239

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtaC

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAW215

Plasmid

#113237

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggAag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW214

Plasmid

#113236

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->gCtag

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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