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pDCAF-AB

Plasmid

#113649

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-AC

Plasmid

#113650

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDCAF-BC

Plasmid

#113651

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAW181

Plasmid

#113127

Purpose

E. coli expression vector for His-MBP-E. coli Cas1 R138A

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Insert

Cas1

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R138A

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pACY.HR4_T7_term_HRR

Plasmid

#111986

Purpose

Subcloning vector position 5

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pACY.HR2_T7_term_HR3

Plasmid

#111984

Purpose

Subcloning vector position 3

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pACY.HR1_T7_term_HR2

Plasmid

#111983

Purpose

Subcloning vector position 2

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pACY.HRF_T7_term_HR1

Plasmid

#111981

Purpose

Sub cloning vector position 1

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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Ternary BAC Reporter

Plasmid

#78220

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBN98

Plasmid

#35133

Depositor

John McCusker

Article

Sinha et al PLoS Genet. 2006 Feb;2(2):e13. Epub 2006

Insert

kanMX4

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pUPD3_CDS_T7pol

Plasmid

#244021

Purpose

GoldenBraid2.0 T7 RNA Polymerase.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

T7pol

Expression

Bacterial

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_CDS_lacI

Plasmid

#244017

Purpose

GoldenBraid2.0 IPTG/Lactose-dependent transcriptional repressor.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_CDS_araC

Plasmid

#244018

Purpose

GoldenBraid2.0 Arabinose-dependent transcriptional activator.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_CDS_tetR

Plasmid

#244019

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-dependent transcriptional repressor.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_CDS_cI857

Plasmid

#244020

Purpose

GoldenBraid2.0 Temperature-dependent transcriptional repressor.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7lac

Plasmid

#243929

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_cI857_PR

Plasmid

#243915

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge