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pFH2.1_mCherry

Plasmid

#205520

Purpose

Express mCherry (without export tag)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mTagBFP2

Plasmid

#234537

Purpose

to label the membrane of cells with a fluorescent protein (mTagBFP2)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mTagBFP2

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJAT74

Plasmid

#204296

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NES (Cytosol)

Plasmid

#220341

Purpose

Mammalian expression vector BS2 in cytoplasm

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NES (Nuclear exclusion sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-p73α-pcDNA3

Plasmid

#22102

Depositor

William Kaelin

Article

Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

Insert

tumor protein p73 (TP73 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180590

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Mutation

PHR(E281A)

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDC180

Plasmid

#218673

Purpose

Expresses rat ⍺SNAP in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

rat ⍺SNAP (Napa Rat)

Tags

6 his

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCGN-Oct-1

Plasmid

#53308

Purpose

plasmid contains POU2F1 (Oct-1) gene with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NLS (Nucleoplasmin)

Plasmid

#220342

Purpose

Mammalian expression vector BS2 in nucleus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NLS (Nucleus localization sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18-GFP

Plasmid

#220495

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

EGFP

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

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pR-spondin 1

Plasmid

#128844

Purpose

bacterial expression of pR-spondin 1

Depositor

Marc de la Roche

Article

Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

Insert

R-spondin 1 (RSPO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPM5

Plasmid

#85189

Purpose

Expresses full-length mouse TRPM5 in mammalian cells

Depositor

Craig Montell

Article

Hofmann et al Curr Biol. 2003 Jul 1;13(13):1153-8.

Insert

TRPM5 (Trpm5 )

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG G-CEPIA1er

Plasmid

#105012

Purpose

ER calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

G-CEPIA1er

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NIK3x (Nucleolus)

Plasmid

#220346

Purpose

Mammalian expression vector BS2 in nucleolus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

Nik3x (Nucleolus localization tag) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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