We narrowed to 10,590 results for: Kars

1. pCIG1_UL136_26kDa_Myc

   Plasmid

   #74955

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

   Expression

   Mammalian

   Mutation

   delta (1-62) M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIG1_UL136_33kda_Myc

   Plasmid

   #74957

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Myc tag (EQKLISEEDL)

   Expression

   Mammalian

   Mutation

   M63A M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

   Plasmid

   #42185

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   G protein Gamma 3(F70A, F71A) (Gng3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   F70A, F71A

   Promoter

   cmv

   Available since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

4. EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

   Plasmid

   #42188

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   Gamma 3(K68A,K69A) (Gng3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K68A,K69A

   Promoter

   cmv

   Available since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

   Plasmid

   #128604

   Purpose

   Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   24xMoonTag
   kinesin family member 18b (KIF18B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7_CC_PE7_IVT_Template

   Plasmid

   #223022

   Purpose

   Template for in vitro transcription of PE7. For HSPC-related experiments.

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUGW-APPSwe-T2A-mCherry

   Plasmid

   #190804

   Purpose

   Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Article

   Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

   Insert

   APP (APP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Swedish (KM670/671NL)

   Promoter

   Ubi C

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE7

   Plasmid

   #214812

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61591

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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