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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin

Plasmid

#84293

Purpose

Expression of wt Merlin

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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HisMBP CFlm25

Plasmid

#78458

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie

Article

Coseno et al Nucleic Acids Res. 2008 Jun;36(10):3474-

Insert

Human Cleavage Factor lm 25 kDa subunit (NUDT21 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRRG757-smedwi-1

Plasmid

#99074

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Smedwi-1

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Smedwi-1 in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG203-notum

Plasmid

#99067

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea notum

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

notum in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

Plasmid

#140226

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRRG272-eya

Plasmid

#99085

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea eyes absent (eya)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

eyes absent (eya)

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-4SS-HEWL

Plasmid

#39233

Depositor

Harald Schwalbe

Article

Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

Insert

Hen egg white lysozyme (LYZ Chicken)

Expression

Bacterial

Mutation

N-terminal Met

Promoter

T7

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pEDA5_GFPmut3_Y66H

Plasmid

#80085

Purpose

Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

Depositor

Timothy Whitehead

Article

Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

Insert

GFPmut3_Y66H

Tags

6x-His tag

Expression

Bacterial

Mutation

changed Tyrosine 66 to Histidine

Promoter

proB

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-delta30-TFEB

Plasmid

#44445

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 1-30; H32D

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-STOP-shA1

Plasmid

#14422

Depositor

Klaus Rajewsky

Article

Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

Insert

modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS2-gfp

Plasmid

#109290

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

WT

Promoter

Kanamycin

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1

Plasmid

#213628

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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CpxR-GFP

Plasmid

#220150

Purpose

To track the natural CpxR promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

CpxR promoter only

Expression

Bacterial

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Article

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Insert

PCP-NLS-GFPEnvy

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160

Plasmid

#100919

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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Syntaxin4-myc-myc-His

Plasmid

#12377

Depositor

Thomas Weimbs

Article

Low et al Mol Biol Cell. 2006 Feb . 17(2):977-89.

Insert

Syntaxin4 (STX4 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Cloned in modified PCDNA4 vector (double myc)

Promoter

CMV⁄TO

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

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