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We narrowed to 14,665 results for: sta

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Showing: 9521 - 9540 of 14665 results

  3. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Publication
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rtTA-Advanced::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199222-plasmid-map-sequence-id-396540

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  5. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Publication
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  6. pKLD-CAG-DYSF-CFL

    Plasmid
    #131457
    Purpose
    Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging
    Depositor
    Michele Calos
    Publication
    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DYSF and Follistatin (DYSF, FST Human)
    Expression
    Bacterial and Mammalian
    Mutation
    R15C (in DYSF) and Q2579H (in CFL)
    Available since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    131457-plasmid-map-sequence-id-258193

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  7. pX459-sg-tdTomato90/816

    Plasmid
    #179918
    Purpose
    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.
    Depositor
    Indeever Madireddy, Johan Sosa
    Publication
    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato sgRNA
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179918-plasmid-map-sequence-id-358501

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  8. pRS405 PEX14-VAC8-RFP

    Plasmid
    #166844
    Purpose
    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF
    Depositor
    Daniel Klionsky
    Publication
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, PEX14 Budding Yeast)
    Tags
    PEX14 transmembrane domain and RFP
    Expression
    Yeast
    Mutation
    PEX14 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166844-plasmid-map-sequence-id-329332

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  9. EC71139

    Plasmid
    #154062
    Purpose
    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)
    Depositor
    Cristobal Uauy
    Publication
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    pZmUbi
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BpiI restriction sites were r…
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154062-plasmid-map-sequence-id-312952

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  12. pHybr_r2a>mG3-srt-his

    Plasmid
    #124805
    Purpose
    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG3
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124805-plasmid-map-sequence-id-244310

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  15. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid
    #127321
    Purpose
    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)
    Depositor
    Jeremy Wilusz
    Publication
    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nanoLuciferase
    Tags
    3xFLAG and CL1/PEST
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    127321-plasmid-map-sequence-id-247733

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  16. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

    Plasmid
    #118622
    Purpose
    expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons
    Depositor
    Marina Chekulaeva
    Publication
    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525
    Insert
    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed ttgcttt to cggtaag (496 - 502 nt in 3…
    Promoter
    synapsin I (rat)
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118622-plasmid-map-sequence-id-230551

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  18. pBV163

    Plasmid
    #108364
    Purpose
    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.
    Depositor
    Scott Moye-Rowley
    Publication
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    Use
    Replace c.glabrata yap1 promoter with c. glabrata…
    Promoter
    TDH3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108364-plasmid-map-sequence-id-209913

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  19. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Publication
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

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  20. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid
    #105813
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105813-plasmid-map-sequence-id-204858

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Showing: 9521 - 9540 of 14665 results