Addgene

We narrowed to 25,751 results for: sta

Showing: 9541 - 9560 of 25751 results

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dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-WIF1

Plasmid

#99533

Purpose

WIF1 expression plasmid

Depositor

Qian Tao

Article

Chan et al Lab Invest. 2007 Jul;87(7):644-50. Epub

Insert

WIF1 (WIF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-STIM2(15-746)

Plasmid

#18868

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

STIM2 (STIM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Signal peptide(SP) 1-10 from STIM1 and residues 1…

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Expression

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-DHB-mTurq2

Plasmid

#212667

Purpose

Express tagged DHB

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

DHB (HELB Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mutation

Promoter

EF-1a

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-18aa-actin

Plasmid

#74259

Purpose

Mammalian expression of N-terminal fusion of mClover3 to actin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Actin

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN PLEKHM1-HA

Plasmid

#89300

Purpose

For expression of PLEKHM1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHsSR beta deltaN Flag

Plasmid

#13731

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRPRB Human)

Use

Tags

6xHis and FLAG

Expression

Bacterial

Mutation

expresses residues 59-271 of Homo sapiens SRβ wit…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pHLmMBP-6

Plasmid

#72346

Purpose

Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Use

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Mutation

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf5

Plasmid

#163596

Purpose

Expresses GDF-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf5 (Gdf5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTrex-Neo-tdTomato

Plasmid

#47975

Purpose

Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

tdTomato

Use

Trypanasoma cruzi expression

Tags

Expression

Mutation

Promoter

T. cruzi rRNA

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin S518A

Plasmid

#84294

Purpose

Expression of Merlin with an alanine mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S518A

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(WT)

Plasmid

#182659

Purpose

Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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peCALWY-1

Plasmid

#22233

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)

Use

Tags

Expression

Mammalian

Mutation

linker consists of 9 GGSGGS repeats

Promoter

Available since

Nov. 18, 2009

Availability

Academic Institutions and Nonprofits only
pHL 1075

Plasmid

#53004

Purpose

pCon:fhlA::mCherry, pLlacO-1:sodB::gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

fhlA
sodB

Use

Synthetic Biology

Tags

GFP and mCherry

Expression

Bacterial

Mutation

contains −309 to +96 bp relative to start codon a…

Promoter

pCon and pLlacO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only