We narrowed to 13,072 results for: BASE

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ipYUC2Cas-LT1

Plasmid

#210559

Purpose

Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1Cas-LT1

Plasmid

#210556

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin

Expression

Plant

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGGC-LexACasPea

Plasmid

#210539

Purpose

Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

LexA::SpCas9::PEA3AT

Use

Synthetic Biology

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGGA-pCLV3

Plasmid

#210528

Purpose

Cloning A. thaliana CLV3 promoter as GreenGate module A

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pCLV3 (CLV3 Mustard Weed)

Use

Synthetic Biology; Golden gate compatible cloning…

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (APEX)

Plasmid

#213406

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-His6

Plasmid

#212776

Purpose

C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-GTS-TurboID

Plasmid

#209397

Purpose

Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

GTS-3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pS44i8GH-2

Plasmid

#207526

Purpose

Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP

Use

CRISPR; Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX173

Plasmid

#202817

Purpose

Plasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

TEV protease

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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