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Plasmid#69936PurposeThe second of the two RNA lassos required for the RNA chain construction.DepositorInsertLasso 2
UseSynthetic BiologyTagsExpressionMutationPromoterTAATACGACTCACTATAGGGAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK501
Plasmid#72246PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationGln206 to Arg and changes tryptophan-151 to pheny…PromoterT7 and endogenousAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E146 Hs.NRAS-nostop
Plasmid#70430PurposeGateway ORF clone of human NRAS [NM_002524.4] without stop codon (for C-terminal fusions)DepositorInsertNRAS (NRAS Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E104 Hs.ICMT-nostop
Plasmid#70388PurposeGateway ORF clone of human ICMT [NM_012405.3] without stop codon (for C-terminal fusions)DepositorInsertICMT (ICMT Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E078 Hs.EXOC6-nostop
Plasmid#70362PurposeGateway ORF clone of human EXOC6 [NM_019053.4] without stop codon (for C-terminal fusions)DepositorInsertEXOC6 (EXOC6 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_3_FRT_+1_OA-
Plasmid#49311PurposeGolden Gate entry vector: Position 3, FRT siteDepositorInsertFRT forward
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_+1_XcmI-LacZ
Plasmid#49295PurposeGolden Gate entry vector at position 1, plus 5' ATG, LacZDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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HP17_KO7
Plasmid#120346PurposeHelper plasmid for production of phagemid ssDNA, based on M13KO7DepositorInsertCoding part of the M13KO7 helper phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA707
Plasmid#186238PurposeKanR-BBR1 entry vector for HR donorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only