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pUdO2c

Plasmid

#210231

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP25-1A(sfGFP)

Plasmid

#126972

Purpose

Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP47-2A(sfGFP)

Plasmid

#126991

Purpose

Level 2 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-proinsulin-mEGFP

Plasmid

#160464

Purpose

Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

proinsulin-mEGFP

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP21-1A(sfGFP)

Plasmid

#126968

Purpose

Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUdO1c

Plasmid

#210230

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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