We narrowed to 1,517 results for: pet-28a

1. pMSP1E1D1

   Plasmid

   #161605

   Purpose

   Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E1D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSP2

   Plasmid

   #26282

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP2
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of dimer of deletion mutant (1-43)…

   Promoter

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pHIT348

   Plasmid

   #204515

   Purpose

   Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   C-terminal fragment of cec-5 (C. elegans)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHIT334

   Plasmid

   #204516

   Purpose

   Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   C-terminal fragment of coh-3 (C. elegans)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHIT186

   Plasmid

   #204513

   Purpose

   Bacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   PAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHIT521

   Plasmid

   #204520

   Purpose

   Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   N-terminal fragment of cec-5 (C. elegans)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHIT182

   Plasmid

   #204511

   Purpose

   Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

   Insert

   csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   D769A

   Promoter

   T7lac

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIT520

   Plasmid

   #204519

   Purpose

   Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   N-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIT342

   Plasmid

   #204514

   Purpose

   Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   N-terminal fragment of syp-1 (C. elegans)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHIT320

    Plasmid

    #204517

    Purpose

    Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen

    Depositor

    Yuji Kohara

    Article

    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

    Insert

    Internal fragment of him-1 (C. elegans) (him-1 Nematode)
    

    Use

    Tags

    6xHis, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSP1E3D1

    Plasmid

    #20066

    Purpose

    Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

    Insert

    MSP1E3D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeats of …

    Promoter

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. eGFP-CBP

    Plasmid

    #169211

    Purpose

    Expression of eGFP-CBP protein for chitin visualization

    Depositor

    James S Fraser

    Article

    Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

    Insert

    Chitinase A1 Chitin Binding Domain
    

    Use

    Tags

    6xHis, Mxe Gyr Intein, and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMSP1D1R1

    Plasmid

    #97323

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1R1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of R residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSP1D1T1

    Plasmid

    #97327

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of T residue after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSP1D1T2

    Plasmid

    #97328

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1T2
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of two T residues after T25

    Promoter

    T7

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Promoter

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NSUN2

    Plasmid

    #188059

    Purpose

    Bacterial expression plasmid for human NSUN2; for activity testing

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge