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pMC-9-35S-11

Plasmid

#176881

Purpose

MoClo Level 0 plasmid, contains 35S

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S terminator

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot8_J

Plasmid

#146699

Purpose

Mammalian Expression of HsNot8

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot8 (CNOT8 Human)

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_5

Plasmid

#172470

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_6

Plasmid

#172471

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1.

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ gcsf

Plasmid

#132947

Purpose

Expresses zebrafish granulocyte colony stimulating factor (csf3a)

Depositor

Jason Berman

Article

Rajan et al Leukemia. 2021 Sep 30. pii: 10.1038/s413

Insert

gcsf (csf3a Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pZS160

Plasmid

#134238

Purpose

Lentiviral plasmid expressing sgRNA against HJURP

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human HJURP sgRNA

Use

Lentiviral

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKM328

Plasmid

#134243

Purpose

Mammalian expression construct expressing sgRNA against PLK1

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

gRNA targeting PLK1 3'

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-TFDP2

Plasmid

#114300

Purpose

Constitutive expression of the TFDP2 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP2 (TFDP2 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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