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PlayBack-U6-Bgl11

Plasmid

#203299

Purpose

For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Not1

Plasmid

#203301

Purpose

For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9

Plasmid

#193862

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Type

Empty backbone

Tags

GFP11, Hisx6, and R9

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-Z _Gogy

Plasmid

#201828

Purpose

Expresses H6_fold-Z _Gogy in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-Z _Gogy

Tags

6xHis

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H7_fold-K _Mussoc

Plasmid

#201830

Purpose

Expresses H7_fold-K _Mussoc in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H7_fold-K _Mussoc

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-U _Nomur

Plasmid

#201829

Purpose

Expresses H6_fold-U _Nomur in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-U _Nomur

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-C _Rei

Plasmid

#201827

Purpose

Expresses H6_fold-C _Rei in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-C _Rei

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#189782

Purpose

Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-RZ-Cas12j2

Plasmid

#189783

Purpose

Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMRBad-C-DiB-RM

Plasmid

#168478

Purpose

C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

C-fragment of DiB-RM

Expression

Bacterial

Promoter

araBAD

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-10

Plasmid

#117039

Purpose

Constitutive integrase cassette - Int5-Int7

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Int5-Int7

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-6

Plasmid

#117035

Purpose

Constitutive integrase cassette - Bxb1-Int5

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Int5

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-7

Plasmid

#117036

Purpose

Constitutive integrase cassette - Bxb1-Int7

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Int7

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-8

Plasmid

#117037

Purpose

Constitutive integrase cassette - Tp901-Int5

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Tp901-Int5

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-9

Plasmid

#117038

Purpose

Constitutive integrase cassette - Tp901-Int7

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Tp901-Int7

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRC03

Plasmid

#114682

Purpose

DHFR-sfGFP.Strand11

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

DHFR-sfGFP.Strand11

Tags

GST

Promoter

T7 Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGGC024

Plasmid

#48829

Purpose

Provides BFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

BFP

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-HtsptLB12

Plasmid

#45122

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

HtsptLB12

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only