We narrowed to 14,168 results for: flag

1. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. IRES-GFP-AXL-KD

   Plasmid

   #65498

   Purpose

   Expresses kinase dead AXL in mammalian cells with cistronic EGFP

   Depositor

   Aaron Meyer

   Article

   Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

   Insert

   Tyrosine-protein kinase receptor UFO (AXL Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K567R

   Promoter

   CMV

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChBtW-iresFUCCI

   Plasmid

   #133371

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-UTY-F

   Plasmid

   #40617

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTY (UTY Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   EF1

   Available Since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pEXP1/Joseph2-Multitagged Plasmid

   Plasmid

   #226724

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pEXP1/Joseph2
   

   Tags

   6xHis, HA, Myc, GFP, Flag, V5

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas9-162A

   Plasmid

   #84918

   Purpose

   for in vitro transcription of Cas9 with polyA tail encoded

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Cas9
   

   Tags

   Flag, NLS, and polyA tail

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 Set9 wt

   Plasmid

   #24082

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA Set9 mutant

   Plasmid

   #24085

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H297A

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

10. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pN1-Lck-GCaMP3

    Plasmid

    #26974

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

    Insert

    Lck-GCaMP3
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. LbdCas12a VPR

    Plasmid

    #188513

    Purpose

    Expresses FLAG tagged LbdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-R4-uAb

    Plasmid

    #101800

    Purpose

    Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF CREB M1

    Plasmid

    #22969

    Depositor

    Marc Montminy

    Article

    Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

    Insert

    CREB (Creb1 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAd-Klf4

    Plasmid

    #19770

    Depositor

    Konrad Hochedlinger

    Article

    Stadtfeld et al Science. 2008 Nov 7;322(5903):945-9. doi

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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