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pScalps_puro_mIL-2Rα RK

Plasmid

#59920

Purpose

Expresses mouse interleukin-2 receptor alpha chain mutated

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Mutation

changed sequence of the intracellular (C-terminal…

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pCSP1-mp53AS-6xMUT

Plasmid

#20904

Depositor

Stefano Piccolo

Article

Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

point mutation (Ser/Thr-Ala) of the 6 N-terminal …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pLAP-MIS12-KARD

Plasmid

#114057

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-10 [N269/73R]

Plasmid

#114558

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-HIPK3 Exon

Plasmid

#69888

Purpose

Expresses the human HIPK3 exon 2 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Fly, Human)

Expression

Insect

Mutation

Expresses aa1-194 of HIPK3

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pD10AH840AhCas9 (GB1041)

Plasmid

#68207

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 CBM

Plasmid

#53163

Purpose

Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

M187A/H188A/M230A/H239A

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5 CBM-E

Plasmid

#53168

Purpose

Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

L280A/H281A/M323A/Q332A

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (G104R)

Plasmid

#29548

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G104R

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
TDP-43_CTD_L330A

Plasmid

#248879

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with L330A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_L330A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed L330A

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_S332A

Plasmid

#248881

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S332A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S332A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S332A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q331A

Plasmid

#248880

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_Q331A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Q331A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q343A

Plasmid

#248889

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q343A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_Q343A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Q343A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q327A

Plasmid

#248878

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q327A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_Q327A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Q327A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_N319A

Plasmid

#248873

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with N319A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_N319A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed N319A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M322A

Plasmid

#248874

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M322A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M322A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M322A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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