We narrowed to 9,832 results for: pET
-
Plasmid#236376PurposeOverexpression of HyperdFnCas12a in human cellsDepositorInsertHyperdFnCas12a
UseCRISPR and LentiviralMutationE184R, T279R, D340R, D409RAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+GFP
Plasmid#233001PurposeGalactose iduced expression of Gcn4 Aro2+GFP in yeastDepositorInsertGcn4 Aro2+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WHA
Plasmid#232995PurposeGalactose iduced expression of Gcn4 ILVtoED W+HAin yeastDepositorInsertGcn4 ILVtoED W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidA
Plasmid#232967PurposeGalactose iduced expression of Gcn4 9acidA in yeastDepositorInsertGcn4 9acidA
TagsTEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+GFP
Plasmid#233002PurposeGalactose iduced expression of Gcn4 Aro3+GFP in yeastDepositorInsertGcn4 Aro3+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WGFP
Plasmid#233007PurposeGalactose iduced expression of Gcn4 LVtoED W+GFPin yeastDepositorInsertGcn4 ILVtoED W+
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RhoA*
Plasmid#198716PurposeExpresses gRNA resistant RhoA under the CAG promoter in mammalian cellsDepositorInsertRhoA (Rhoa Mouse)
ExpressionMammalianMutationIt has mutations that is resistant to the RhoA gR…PromoterCAGAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSN687
Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
TagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ
Plasmid#172111PurposeExpression of Methionine Binding Protein (MetQ) with signal sequenceDepositorInsertMethionine Binding Protein
Tags10x Histidine Tag and N.meningitidis signal seque…ExpressionBacterialMutationChanged Proline 22 to AlaninePromoterT7 PromoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _2-33/myc-His
Plasmid#127492PurposeExpresses myc-tagged _MTS NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _2-33 (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 _2-33PromoterCMVAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2 _2-78/myc-His
Plasmid#127493PurposeExpresses myc-tagged _2-78 NDUFS2 in mammalian cellsDepositorInsertNDUFS2 _2-78 (NDUFS2 Human)
Tagsmyc-HisExpressionMammalianMutationNDUFS2 _2-78PromoterCMVAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
Tags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
Tags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available SinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_proNR2_LGK.9
Plasmid#81162PurposeExpresses levoglucosan kinase variant LGK.9 with promoter proNR2DepositorInsertLevoglucosan kinase
TagsHis6xExpressionBacterialMutationLGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…PromoterproNR2Available SinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK500
Plasmid#72245PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and endogenousAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1073
Plasmid#53003PurposepCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsompC (target mRNA) and sodB (target mRNA)ExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon and pLlacO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 3545 (=HL 770 + pHL 1213)
Plasmid#30081DepositorInsertpLlacO-1:hfq, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsGFPExpressionBacterialAvailable SinceJune 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-E222A-E272A
Plasmid#35060DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
TagsHisMutationContains HinA domain: aa 192-393; E222A and E272A…PromoterT7Available SinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA-Y218A-Y267A
Plasmid#35059DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
TagsHisMutationContains HinA domain: aa 192-393; Y218A and Y267A…PromoterT7Available SinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only