We narrowed to 12,188 results for: LEA
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Plasmid#212976PurposeEncodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertEnterovirus A71 4643 VP12A C110A
UseMammalian cell free protein expressionTagsHA tagMutationchanged cysteine 110 from 2A to alaninePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A_C109R
Plasmid#212987PurposeEncodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney VP12A C109R
UseMammalian cell free protein expressionTagsHAMutationCysteine 109 from 2A mutated to argininePromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
ExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_g5-HT2m
Plasmid#208716PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in neuronsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
UseAAVPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2m
Plasmid#208710PurposeExpresses the green 5-HT sensor GRAB_g5-HT2m in mammalian cellsDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2m
ExpressionMammalianPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_CRF0.5
Plasmid#208660PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A
Plasmid#195707PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domainDepositorInsertSyt9 (Syt5 Mouse)
TagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2B
Plasmid#195708PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2B domainDepositorInsertSyt9 (Syt5 Mouse)
TagsGSTExpressionBacterialMutationencodes amino acids 253-344 of mouse SYT9Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2AB
Plasmid#195709PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2AB domainDepositorInsertSyt9 (Syt5 Mouse)
TagsGSTExpressionBacterialMutationencodes amino acids 104-386 of mouse SYT9Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
TagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1Available sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKP2del4
Plasmid#130837PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acid residuesPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2delKASH
Plasmid#130838PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
TagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domain, addition of three alaniā¦PromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
PET-HeFSpCas9-NLS-6xHis
Plasmid#207391PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHeFSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060APromoterT7Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_S4.39U>G.51A>C
Plasmid#204173PurposepRegev_S4 with double mutations that restore secondary structure.DepositorInsertExon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene
UseSynthetic BiologyExpressionMammalianMutation39U>G and 51A>C mutationsPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Ctdnep1_C-ter
Plasmid#196525PurposeExpression of Ctdnep1_C-terDepositorInsertCtdnep1-C-ter (CTDNEP1 Human)
TagsHisExpressionBacterialMutationNo transmembrane domainPromoterT7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
hTln1-R1R2
Plasmid#191440PurposeExpresses the human TLN1 R1R2 domains in bacteriaDepositorAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FTS
Plasmid#198526PurposeExpression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)DepositorAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-Bio2_MVPI_SMAC(delta(55))_9His
Plasmid#197978PurposeE. coli expression of MVPI-SMAC(delta(55))_9HisDepositorAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-Bio2_SMAC(delta(55))_Cys_9His
Plasmid#197977PurposeE. coli expression of SMAC(delta(55))_Cys-9HisDepositorAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only