We narrowed to 12,202 results for: 112

1. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

   Plasmid

   #176626

   Purpose

   TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFL-SV40-p38 β/MAPK11 5'-UTR

   Plasmid

   #115357

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   p38 β/MAPK11 5'-UTR (MAPK11 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p23-puro-EGFP-FBL

   Plasmid

   #179235

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   FBL (FBL Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

   Plasmid

   #194691

   Purpose

   hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCpf1-eBE

   Plasmid

   #107688

   Purpose

   Expresses dLbCpf1-eBE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE
   

   Expression

   Mammalian

   Mutation

   dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mRuby3-MLC-IRES-Neo

   Plasmid

   #222635

   Purpose

   Reporter for activated NM2

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mRuby3-MLC (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Promoter

   EF1a

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ChRmine-eYFP-WPRE

   Plasmid

   #130992

   Purpose

   AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Promoter

   hSyn

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag WDR24 pRK5

   Plasmid

   #46334

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   WDR24 (WDR24 Human)
   

   Tags

   FLag

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-MECP2

   Plasmid

   #47806

   Purpose

   Expresses methyl-CpG binding protein 2 (Rett Syndrome)

   Depositor

   Huda Zoghbi

   Article

   Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

   Insert

   Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
   

   Use

   Gateway entry vector

   Mutation

   wild type

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pNIC28-Bsa4_ZV-NS5-RdRp

    Plasmid

    #179847

    Purpose

    The RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89

    Depositor

    Julien Lescar

    Article

    Gharbi-Ayachi et al J Virol. 2020 Oct 14;94(21). pii: JVI.00

    Insert

    Zika Virus Non-structural protein 5 RdRp domain
    

    Tags

    6-histine

    Expression

    Bacterial

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SNAP-BAF

    Plasmid

    #227443

    Purpose

    Mammalian expression of SNAP-BAF

    Depositor

    David Pellman

    Article

    Umbreit et al Science. 2020 Apr 17;368(6488):eaba0712.

    Insert

    Barrier-to-autointegration factor (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX FLAG-SLC38A9.1

    Plasmid

    #199749

    Purpose

    Lentiviral construct to stably express SLC38A91.1

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    SLC38A9.1
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENN491_TALEcharm_MmT8x

    Plasmid

    #220836

    Purpose

    TALEcharm Kv2 expression in mammalian cells; targets Prnp

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    TALEcharm
    

    Use

    Lentiviral and TALEN

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40

    Plasmid

    #194692

    Purpose

    hSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3.7m-psMEK2

    Plasmid

    #89363

    Purpose

    A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK2 (MAP2K2 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. S1PR1-DuET

    Plasmid

    #213374

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR1 (S1PR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUW-tetO-Stat3

    Plasmid

    #139805

    Purpose

    doxycycline-inducible expression of mouse Stat3 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p23-mNeongreen-DDX21 WT-mRuby3

    Plasmid

    #179225

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX462-hPRKAA1-gRNA_B

    Plasmid

    #74375

    Purpose

    gRNA_B to knockout human AMPK alpha 1 using Cas9n

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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