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pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231353

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

Plasmid

#231356

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

Plasmid

#231357

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

Plasmid

#231358

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

Plasmid

#231359

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

Plasmid

#231348

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228373

Purpose

Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228377

Purpose

Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_ProRS_3278.3.7_SHI28108_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228379

Purpose

Expresses N-term transit peptide from Sapria himalayana Proline aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA); determination of S. himalayana ProRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana ProRS evm.model.fragScaff scaffold 3278.3.7 (SHI28108RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Athal_PheRS_TP_pK7fWG2_IVD_FP611

Plasmid

#228370

Purpose

Expresses N-term transit peptide from Arabidopsis PheRS At3g58140 with demonstrated dual mito/plastid targeting; proof-of-concept for Plasmid 228371

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from Arabidopsis PheRS At3g58140 (Warren et al., 2023) with demonstrated dual mito/plastid targeting.

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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LCV2 PQBP1 KO2

Plasmid

#217448

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA Q63/67A

Plasmid

#217439

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q63A,Q67A

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2 PQBP1 KO1

Plasmid

#217447

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-α-Synuclein-c_myc

Plasmid

#225222

Purpose

Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

α-Synuclein
Aga2

Tags

GS linker, HA Tag, and c-myc Tag

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge