We narrowed to 25,837 results for: lars

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GB4652

Plasmid

#215266

Purpose

TU for the copper regulated expression of the flippase (FLP) recombinase.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS:miniDFR:FLP:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB1203

Plasmid

#215369

Purpose

TU for the expression of the silencing suppressor P19 driven by the 35s promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

35s:P19:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ223

Plasmid

#217497

Purpose

Bacterial expression of BaFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc guide RNA scaffold

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ224

Plasmid

#217496

Purpose

Bacterial expression of BaFnuc for IVTT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc

Expression

Bacterial

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 N-terminal sgRNA

Plasmid

#207094

Purpose

pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTACTGCAGAATGACTACAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD1 N-terminal sgRNA

Plasmid

#207101

Purpose

pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATGGCAGGACTATGGCAGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM C-terminal sgRNA

Plasmid

#207097

Purpose

pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTCTAAAGGCTGAATGAAA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP)

Plasmid

#218640

Purpose

Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 hNLRP3 R262W (disease-associated mutation)

Plasmid

#218647

Purpose

Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Gateway entry

Mutation

R262W

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 msNLRP3(ΔPYD, I125M-END)

Plasmid

#218641

Purpose

Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Gateway entry

Mutation

delPYD (I125M-END)

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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