We narrowed to 57,317 results for: plasmid
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Plasmid#119765PurposeLentiviral plasmid for Mammalian ExpressionDepositorInsertAID-Dam only
UseLentiviralExpressionMammalianAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human 3' HP1a AID GFP PuroR
Plasmid#127906PurposeHDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a GeneDepositorInsertHP1 (CBX5 Human, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRMutationInserting GFP AID 2A Puro into mouse 3' Hp1aAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-hygro-2xMyc-Rac1-WT
Plasmid#128580PurposeFor mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.DepositorAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
Plasmid#101300PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.DepositorInsertalpha-Catenin
ExpressionMammalianMutationContains amino acids 1-509, 670-698, and 699-906 …Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGT-Ah7
Plasmid#122574PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah10
Plasmid#122577PurposeMAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)DepositorInserttetQ/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah3
Plasmid#122570PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-YU2gp160
Plasmid#100927PurposeMammalian expression plasmid for Env from the YU-2 HIV-1 isolateDepositorInsertHIV-1 (YU2) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-FBFP
Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ER-moxMaple3
Plasmid#120885Purposemammalian expression of ER localized moxMaple3DepositorInsertmoxMaple3
TagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianPromoterCMVAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-3R
Plasmid#112091PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.DepositorAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH3(2)-4R
Plasmid#112090PurposeBacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.DepositorAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR
Plasmid#119859PurposegRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporterDepositorInsertgRNA dual luciferase reporter
UseCRISPRExpressionMammalianAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)
Plasmid#114081PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.1DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
TagsSV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)
Plasmid#114082PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)
Plasmid#114068PurposeT7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guidesDepositorInsertentry vector for LbCas12a crRNAs
UseIn vitro transcriptionAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239
Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MAC_C_AIFM1
Plasmid#111659PurposeMAC-tagged gene expressionDepositorInsertAIFM1 (AIFM1 Human)
ExpressionMammalianAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only