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pLKO-Cre sgItgb5 v3

Plasmid

#158038

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Itgb5 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgRipk4 v3

Plasmid

#158035

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-Mm_Plk5(D168A)

Plasmid

#136318

Purpose

Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Expression

Mammalian

Mutation

D168A

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Hu_Plk5(W207F)

Plasmid

#136320

Purpose

Mammalian expression of human Plk5 W207F with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Expression

Mammalian

Mutation

W207F

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-Dronpa

Plasmid

#138250

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-Dronpa

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ctcf-tdiRFP670-PEST

Plasmid

#122357

Purpose

Targeting vector for mouse Ctcf to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ctcf

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3l-tdiRFP670-PEST

Plasmid

#122358

Purpose

Targeting vector for mouse Dnmt3l to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3l

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Etv5-tdiRFP670-PEST

Plasmid

#122359

Purpose

Targeting vector for mouse Etv5 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Etv5

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Jade1-tdiRFP670-PEST

Plasmid

#122360

Purpose

Targeting vector for mouse Jade1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Jade1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Kdm1a-tdiRFP670-PEST

Plasmid

#122361

Purpose

Targeting vector for mouse Kdm1a to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Kdm1a

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ncapd3-tdiRFP670-PEST

Plasmid

#122362

Purpose

Targeting vector for mouse Ncapd3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ncapd3

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Npm1-tdiRFP670-PEST

Plasmid

#122363

Purpose

Targeting vector for mouse Npm1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Npm1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3b-tdiRFP670-PEST

Plasmid

#122364

Purpose

Targeting vector for mouse Dnmt3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dhx15-tdiRFP670-PEST

Plasmid

#122365

Purpose

Targeting vector for mouse Dhx15 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dhx15

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Birc6-tdiRFP670-PEST

Plasmid

#122366

Purpose

Targeting vector for mouse Birc6 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Birc6

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Trim28-tdiRFP670-PEST

Plasmid

#122367

Purpose

Targeting vector for mouse Trim28 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Trim28

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Gsk3b-tdiRFP670-PEST

Plasmid

#122368

Purpose

Targeting vector for mouse Gsk3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Gsk3b

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Cdt1-tdiRFP670-PEST

Plasmid

#122369

Purpose

Targeting vector for mouse Cdt1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Cdt1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ddx42-tdiRFP670-PEST

Plasmid

#122370

Purpose

Targeting vector for mouse Ddx42 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ddx42

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-L

Plasmid

#122343

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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