We narrowed to 12,202 results for: 112

1. Chr7_Centromere-Targeting_gRNA

   Plasmid

   #195129

   Purpose

   dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Chr7 gRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. YCplac33-NAT_MCART1

   Plasmid

   #140591

   Purpose

   Express MCART1 in S. cerevisiae

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
   

   Expression

   Yeast

   Mutation

   codon-optimized for expression in S. cerevisiae

   Promoter

   NDT1 promoter and 3'UTR

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hPPP3CC-FLAG

   Plasmid

   #179162

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3/TO/V5-DEST-IGFBP2

   Plasmid

   #191006

   Purpose

   Lentiviral expression vector for IGFBP2

   Depositor

   Johanna Ivaska

   Article

   Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

   Insert

   Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-GFP-MCC

   Plasmid

   #133307

   Purpose

   "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   microcin-V bacteriocin cassette (cvaC E. coli)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N112D (please see depositors comments)

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX462-hPRKAA1-gRNA_A

   Plasmid

   #74374

   Purpose

   gRNA_A to knockout human AMPK alpha 1 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

   Plasmid

   #78251

   Purpose

   Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -loxP-mTagBFP2-stop-
   mCherry-NS-MCS
   

   Use

   Zebrafish motor neuron inducible expression

   Promoter

   -3mnx1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR25-DuET

   Plasmid

   #213277

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR25 (GPR25 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. plentiCRISPv2_AAVS1

    Plasmid

    #184487

    Purpose

    Lentivirus all in one CRISPR vector targeting human AAVS1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    AAVS1 (AAVS1 Human)
    

    Use

    Lentiviral

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-BG505.SOSIP-gp160-754*

    Plasmid

    #123274

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncation

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-BG505.SOSIP-TM

    Plasmid

    #111844

    Purpose

    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Tags

    CD5 leader sequence and TM helix from MHC class I

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

    Plasmid

    #153308

    Purpose

    Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

    Depositor

    Marcel Bruchez

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    IgKappa-myc-dL5-2XG4S-GPI
    

    Tags

    myc-dL5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

    Plasmid

    #221224

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-eGFP-Ago2

    Plasmid

    #74231

    Purpose

    GFP-Ago2 lentiviral vector

    Depositor

    Carl Novina

    Article

    Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. A3G-BE5.14

    Plasmid

    #163638

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE5.14
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid

    #191334

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. A3G-BE5.13

    Plasmid

    #163637

    Purpose

    C to T DNA base editing

    Depositor

    Xue Gao

    Article

    Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

    Insert

    A3G-BE5.13
    

    Tags

    BPSV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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