We narrowed to 13,211 results for: CAR

1. pscALPSpuro-RsACE2

   Plasmid

   #159665

   Purpose

   Expresses Rhinolophus sinicus ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNA2.0 6H-TEV-KRAS G12V

   Plasmid

   #111850

   Purpose

   Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

   Depositor

   Kenneth Westover

   Article

   Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

   Insert

   KRAS G12V (KRAS Human)
   

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Mutation

   Changed Glycine 12 to Valine

   Promoter

   T5

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #84480

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Flag-CDK19-WT-IRES-ZsGreen

   Plasmid

   #68858

   Purpose

   Expresses Human CDK19 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #153536

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   Syn

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234450

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65454

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

   Plasmid

   #112677

   Purpose

   An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Nuclear-localized floxed-mCherry EGFP
   

   Use

   AAV

   Tags

   Nuclear localization signal

   Promoter

   EF1a

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_HA.V5.VSVg_NGFR

   Plasmid

   #158244

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.V5.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT1-2A-eGFP PGK PuroR Donor Plasmid

    Plasmid

    #82333

    Purpose

    WT1 targeting donor

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Inserts

    WT1 5' Arm (WT1 Human)
    WT1 3' Arm (WT1 )
    

    Use

    CRISPR and TALEN

    Tags

    2A-eGFP

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. rag2:FRS2_CE_pISceI

    Plasmid

    #223040

    Purpose

    Express FRS2 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    FRS2 (FRS2 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-SNAP-hCB2_IRES_EGFP

    Plasmid

    #223506

    Purpose

    Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

    Depositor

    James Frank

    Article

    Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

    Insert

    SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)
    

    Tags

    SNAP-tag

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid

    #114085

    Purpose

    CAG promoter expression plasmid for human codon optimized LbBE1.1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D832A

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv1-sgPHGDH-G5

    Plasmid

    #83913

    Purpose

    stable knockout

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBFC0996

    Plasmid

    #186695

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    crRNA
    Tn7 transposon
    2xLguI Cargo Stuffer
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHC068 BRD2-KI-GFP-2A-L387A

    Plasmid

    #231713

    Purpose

    CRISPR mediated KI of BromoTag at N-terminus of BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065

    Depositor

    Alessio Ciulli

    Article

    Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

    Insert

    Brd2 (BRD2 Human)
    

    Use

    CRISPR

    Tags

    BromoTag and GFP

    Expression

    Mammalian

    Mutation

    L387A

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX304_zeo_mmFbxw7a

    Plasmid

    #160102

    Purpose

    Expresses murine Fbxw7 alpha in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInducer10b-HA-KRAS WT CO SR

    Plasmid

    #164926

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Mutation

    9 Valine codons are optimized and siRNA targetin…

    Promoter

    Ubc promoter

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgTP53(R273)

    Plasmid

    #85561

    Purpose

    Expresses sgRNA targeting human TP53 around codon R273

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only