We narrowed to 161,813 results for: addgene

1. pJM103: 150bp Hps2 RFP on pCM66T

   Plasmid

   #74744

   Purpose

   pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pHps2, trimmed (extracted from Methylovorus gluco…

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GDAP2

   Plasmid

   #73259

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NM_001135589.2 (GDAP2 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. 5C0M

   Plasmid

   #69962

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SGF29:JMC01N-E01:C207060 (SGF29 Human)
   

   Use

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pPyCAG-Nanog(Q124A)-IP

   Plasmid

   #62993

   Purpose

   Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q124A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 124 (125 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPyCAG-Nanog(M125A)-IP

   Plasmid

   #62994

   Purpose

   Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog M125A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Methionine 125 (126 AA in Mouse Protein) …

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPyCAG-Nanog(Q138A)-IP

   Plasmid

   #62997

   Purpose

   Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q138A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 138 (139 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPyCAG-Nanog(T141A)-IP

   Plasmid

   #62999

   Purpose

   Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog T141A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Threonine 141 (142 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPyCAG-Nanog(Q144A)-IP

   Plasmid

   #63000

   Purpose

   Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog Q144A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Glutamine 144 (145 AA in Mouse Protein) t…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPyCAG-Nanog(N145A)-IP

   Plasmid

   #63001

   Purpose

   Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

   Depositor

   Shinya Yamanaka

   Insert

   Mouse Nanog N145A Mutant (Nanog Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Asparagine 145 (146 AA in Mouse Protein) …

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPyCAG-Nanog(R147A)-IP

    Plasmid

    #63002

    Purpose

    Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog R147A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Arginine 147 (148 AA in Mouse Protein) to…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPyCAG-Nanog(K151A)-IP

    Plasmid

    #63004

    Purpose

    Expresses Mouse Nanog K151A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K151A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Lysine 151 (152 AA in Mouse Protein) to A…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPyCAG-Nanog(T100A)-IP

    Plasmid

    #62989

    Purpose

    Expresses Mouse Nanog T100A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog T100A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Threonine 100 (101AA) to Alanine

    Promoter

    CAG

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPyCAG-Nanog(F102A)-IP

    Plasmid

    #62990

    Purpose

    Expresses Mouse Nanog F102A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog F102A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Phenylalanine 102 (103AA in Mouse Protein…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTF257 (pFA6a-TEV-6xGly-His3MX)

    Plasmid

    #44068

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTF258 (pFA6a-PSc-6xGly-His3MX)

    Plasmid

    #44062

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid

    #44080

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid

    #44074

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid

    #44092

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PP-MAPK2 (3RP9)

    Plasmid

    #32862

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MAPK2
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    amino acids 123-548 only, P421L

    Promoter

    Available since

    Jan. 10, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

20. MPP8 (3R93)

    Plasmid

    #32050

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MPP8 (MPHOSPH8 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits