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pDSM-ab-Psptef1-NAT-Ttip1

Plasmid

#127747

Purpose

Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

nourseothricin N-acetyltransferase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptef1

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNS3_Ptrc::luxR _Plux::Cdv3/GFP

Plasmid

#239009

Purpose

Cell elongation, under LuxR control, activation by 3OC6-HSL and 3OC12-HSL

Depositor

Daniel Ducat

Article

Kokarakis et al Metab Eng. 2025 Nov;92:76-89. doi: 10.10

Inserts

Transcriptional activator luxR
DivIVA-like cell division regulating protein

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Promoter

PluxI and Ptrc

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-RSV-mStayGold

Plasmid

#241381

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with RSV promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

RSV

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CBh-mStayGold

Plasmid

#241390

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

CBh

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-PGK-mStayGold

Plasmid

#241392

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-hEF1a-mStayGold

Plasmid

#241377

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

hEF1a

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-ShortCAG-mStayGold

Plasmid

#241385

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

ShortCAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-PGK-mStayGold

Plasmid

#241384

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_UbC

Plasmid

#241375

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-SV40-mStayGold

Plasmid

#241382

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mStayGold

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1-I87L

Plasmid

#232749

Purpose

IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

I87L

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_TRE-jRCaMP1b

Plasmid

#229798

Purpose

Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

jRCaMP1b

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFNC-6

Plasmid

#227669

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Inserts

BxbI phage integrase
sacB

Expression

Bacterial

Promoter

PEM7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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(J) crtYB(K) G3 (SBE117)

Plasmid

#194996

Purpose

Rhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(J) crtYB(K)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(G) crtI (H) G2 (SBE116)

Plasmid

#194995

Purpose

Rhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(G) crtI (H)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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(D) crtE (E)_G1 (SBE115)

Plasmid

#194994

Purpose

Rhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(D) crtE (E)

Expression

Bacterial

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDA2ScIfm

Plasmid

#193786

Purpose

Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTATAA synthetic promoter carrying binding sites…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTA1cI-T3

Plasmid

#193789

Purpose

Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Truncated version of Lambda CI, named T3, where the first two codons are deleted

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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