We narrowed to 31,881 results for: Eng

1. pDSM-ef-Psbtdh3-CSC-Tefm1

   Plasmid

   #127742

   Purpose

   Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   citrate synthase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Psbtdh3

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSM-f3-Peno2-ACDH-Ttal1

   Plasmid

   #127743

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Peno2

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSM-f3-Pvma6-ACDH-Trps14a

   Plasmid

   #127744

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pvma6

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSM-f3-Prpl28-ACDH-Tecm10

   Plasmid

   #127745

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Prpl28

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSM-f3-Ptdh3-ACDH-Trpl3

   Plasmid

   #127746

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Ptdh3

   Availability

   Academic Institutions and Nonprofits only

   

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6. eeBxb1

   Plasmid

   #222339

   Purpose

   Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Nme2ABE8e

   Plasmid

   #189928

   Purpose

   Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   Nme2ABE8e
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CjABE8e

   Plasmid

   #189929

   Purpose

   Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   CjABE8e
   

   Expression

   Mammalian

   Mutation

   CjCas9 D8A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. wtBxb1

   Plasmid

   #222337

   Purpose

   Plasmid for expressing wild type Bxb1 in mammalian cells

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized wild type Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAVS1 pegRNA1 with trimmed attP

    Plasmid

    #222346

    Purpose

    pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA1
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. eeBxb1_IVT

    Plasmid

    #222342

    Purpose

    Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Template for in vitro transcription

    Promoter

    T7 (inactivated)

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. aeBlue chromoprotein

    Plasmid

    #117846

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, aeBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. fwYellow chromoprotein

    Plasmid

    #117841

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, fwYellow
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBEI-6409

    Plasmid

    #47048

    Purpose

    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    

    Use

    Synthetic Biology; Bglbrick

    Expression

    Bacterial

    Promoter

    PlacUV5

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS3Clux

    Plasmid

    #55172

    Purpose

    lux-reporter vector, integration at sacA, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. amilCP_Pink chromoprotein

    Plasmid

    #117851

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Pink
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    64A,65C, BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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