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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-ARshRNA1

Plasmid

#220561

Purpose

To inducibly knockdown AR expression

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

AR shRNA1 (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G (TetOP) promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX VAMP7 (1-188)

Plasmid

#42315

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Δ181-220

Promoter

Ptac

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-eEF2K(S441A/S445A)

Plasmid

#110161

Purpose

Expression of human eEF2K (S441A/S445A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S441A/S445A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (1-120)

Plasmid

#42317

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Longin domain (1-120) (VAMP7 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Δ120-220

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-YFP

Plasmid

#29593

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-P525L-YFP

Plasmid

#29628

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

P525L

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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426Gal-FUS

Plasmid

#29591

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
LentiRCas9-Lambda2

Plasmid

#104184

Purpose

Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Article

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-sgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL

Plasmid

#160286

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-Bat-CoV-HKU9-3CL

Plasmid

#160284

Purpose

Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF3185

Plasmid

#144661

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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416Gal-FUS-R521C-YFP

Plasmid

#29622

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R521C

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLEX307-SARS-CoV-3CL

Plasmid

#160280

Purpose

Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-CUL4A K705R-P2A-Puro

Plasmid

#124305

Purpose

Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed Lysine 705 to Arginine

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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FusionRed-Fibrillarin-7

Plasmid

#56118

Purpose

Localization: Nucleoli, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Fibrillarin (FBL Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
416Gal-FUS-R521G-YFP

Plasmid

#29623

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R521G

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only