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We narrowed to 1,518 results for: pet-28a

Showing: 961 - 980 of 1518 results

  2. pSG086

    Plasmid
    #214263
    Purpose
    PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)
    Depositor
    Ute Romling
    Article
    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi
    Insert
    Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
    Use
    Tags
    6x His-tag
    Expression
    Bacterial
    Mutation
    substitution of 31 amino acids of FtsH2 (F124RRFA…
    Promoter
    T7 promoter
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214263-plasmid-map-sequence-id-447733

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  3. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  5. HIS_TEV_hsSYX3

    Plasmid
    #179290
    Purpose
    E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7lac
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179290-plasmid-map-sequence-id-354913

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  6. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Melissa Jurica, Tara Davis
    Insert
    ppie (PPIE Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Promoter
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  7. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

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  15. pH6-2xmNeonC

    Plasmid
    #178463
    Purpose
    A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.
    Depositor
    Siegfried Musser
    Article
    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:
    Insert
    mNeonGreen dimer
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Free cysteine at the C-terminus of the dimer
    Promoter
    T7
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    178463-plasmid-map-sequence-id-392808

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  16. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  17. pSNRW-Z3-Nluc

    Plasmid
    #174486
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z3-Nluc
    Use
    Tags
    6x Histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174486-plasmid-map-sequence-id-345289

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  18. pSNRW-Z5-Nluc

    Plasmid
    #174487
    Purpose
    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
    Depositor
    Richard C. Willson
    Article
    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1
    Insert
    Z5-Nluc
    Use
    Tags
    6x Histidine tag (N terminal on insert)
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available Since
    Oct. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    174487-plasmid-map-sequence-id-345291

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  19. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  20. His-DNPH1

    Plasmid
    #203224
    Purpose
    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    DNPH1 (DNPH1 Human)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203224-plasmid-map-sequence-id-400075

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Showing: 961 - 980 of 1518 results