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pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)

Plasmid

#223748

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)

Plasmid

#223749

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

expresses aa 1-465, with W276A/I279A; deletion of…

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR)

Plasmid

#223750

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

FUNDC1 soluble part (FUNDC1 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

expresses aa 1-50, with Y18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223753

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

expresses aa 1-182, with E72A/L75A/D77A/E81A; del…

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (450-517)-ULK1 (836-1050)-His

Plasmid

#222825

Purpose

Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His and MBP

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His and MBP

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#222822

Purpose

Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

6His-GST

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

Plasmid

#222821

Purpose

Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

TwinStrep Flag and msfGFP-10His

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF

Plasmid

#203549

Purpose

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)

Plasmid

#203548

Purpose

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#203547

Purpose

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Tags

TwinStrep Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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HH13 Clone 13 heavy chain

Plasmid

#192177

Purpose

Clone 13 heavy chain (HH013)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13 heavy chain (HH013)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HH13A Clone 13A heavy chain

Plasmid

#192178

Purpose

Clone 13A heavy chain (HH13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A heavy chain (HH13A)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HK13A Clone 13A light chain

Plasmid

#192182

Purpose

Clone 13A light chain (HK13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A light chain (HK13A)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ288 (BoCas12a)

Plasmid

#138125

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

BoCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ283 (MlCas12a)

Plasmid

#138115

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MlCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ285 (Lb5Cas12a)

Plasmid

#138120

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Lb5Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ286 (CMaCas12a)

Plasmid

#138122

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

CMaCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge