We narrowed to 15,159 results for: nts

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pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

Plasmid

#139994

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF5_WT

Plasmid

#82167

Purpose

Gateway Donor vector containing TRAF5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF5 (TRAF5 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpG-P2A-EGFP (RTW4177)

Plasmid

#139988

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTRClover

Plasmid

#59151

Purpose

Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-p300

Plasmid

#191764

Purpose

Expression of EGFP fused to core p300 histone acetyltransferase

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (EP300 Human)

Tags

EGFP (N-terminal of p300 core) and FRB (N-termina…

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Article

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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p80-Katanin-mNeonGreen

Plasmid

#191762

Purpose

Expression of mNeonGreen fused to p80-Katanin

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

p80-Katanin-mNeonGreen (KATNB1 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEN243-EN1953

Plasmid

#224546

Purpose

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

Plasmid

#170240

Purpose

Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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RUSH reporter-HMGB1-SBP-GFP

Plasmid

#172357

Purpose

Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

Depositor

Guido Kroemer

Article

Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

Insert

high mobility group box 1 (HMGB1 Human)

Use

Lentiviral

Tags

SBP-GFP

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Promoter

human ubiquitin C

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

Plasmid

#140005

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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EB3-mNeonGreen

Plasmid

#191761

Purpose

Expression of mNeonGreen fused to EB3

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

EB3-mNeonGreen (MAPRE3 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

Plasmid

#170220

Purpose

Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Mutation

T14A, Y15F

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKMW123_SpRY-Cas9_U6-entry

Plasmid

#244820

Purpose

Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

Plasmid

#187320

Purpose

dCas9 expression vector with cloning site for C terminal fusions

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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