We narrowed to 13,211 results for: CAR

1. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

   Plasmid

   #84483

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

   Plasmid

   #114095

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (E174R/S542R/K607R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R, S542R and K607R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

   Plasmid

   #114086

   Purpose

   CAG promoter expression plasmid for human codon optimized LbBE1.4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Mutation

   D10A

   Promoter

   Mecp2

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgTP53(A)

   Plasmid

   #85559

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pLVX-Flag-CDK19-W105M-IRES-ZsGreen

   Plasmid

   #68859

   Purpose

   Expresses Human CDK19 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-FLEx-iSeroSnFR

   Plasmid

   #128486

   Purpose

   Fluorescent reporter for serotonin (conditional + membrane localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, PDGFR, and cpsfGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128589

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   Syn

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLXI_TRC403 LacZ

   Plasmid

   #111184

   Purpose

   Control inducible vector with LacZ (V5 tagged)

   Depositor

   William Hahn

   Article

   Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

   Insert

   lacZ
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   TRE

   Available Since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid

    #110325

    Purpose

    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLXI_TRC401 LacZ

    Plasmid

    #111183

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

    Plasmid

    #47633

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2 (H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    EF1a

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GFAP-eGFP-shAqp4-4

    Plasmid

    #223225

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-GFAP-eGFP-shAqp4-3

    Plasmid

    #223224

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-GFAP-eGFP-shAqp4-1

    Plasmid

    #223223

    Purpose

    mir30 based shRNA strategy, shRNA targeting mouse Aqp4

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shAqp4 (Aqp4 Mouse)
    

    Use

    AAV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-832_NY-ESO-1_TCR_FAS/4-1BB

    Plasmid

    #207495

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/4-1BB, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

    Plasmid

    #163179

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-835_NY-ESO-1_TCR_FAS/MyD88

    Plasmid

    #207498

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/MyD88, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPRv1-sgAAVS1

    Plasmid

    #83906

    Purpose

    stable knockout

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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