We narrowed to 11,368 results for: VARS

1. pARR5::LUC

   Plasmid

   #141170

   Purpose

   Luminescent reporter for cytokinin signaling

   Depositor

   Vardis Ntoukakis, Patrick Schäfer

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtARR5 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtARR5, AT3G48100

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Catharine Ross, Reza Zolfaghari

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG:: ChR2HA-2a-hM4D

   Plasmid

   #52520

   Purpose

   Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Insert

   ChR2-2a-hM4D
   

   Tags

   2xHA on ChR2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-RSET-iGABASnFR2n-6xHis

   Plasmid

   #218886

   Purpose

   Bacterial expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S99A F104H R168P

   Promoter

   T7

   Available Since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAB18::LUC

   Plasmid

   #141168

   Purpose

   Luminescent reporter for abscisic acid signaling

   Depositor

   Vardis Ntoukakis, Patrick Schäfer

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtRAB18 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtRAB18, AT5G66400

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30 GLTP

   Plasmid

   #170733

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

   Insert

   GLTP (GLTP Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC34

   Plasmid

   #63915

   Purpose

   Recipient overexpressing vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Tags

   mCherry

   Expression

   Yeast

   Promoter

   TEF1 (Saccharomyces cerevisiae)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hRedOpsin-TGFB2

   Plasmid

   #164428

   Purpose

   AAV plasmid expressing TGFB2 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

   Insert

   Tgfb2 (Tgfb2 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human red opsin

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pES134

    Plasmid

    #127551

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Article

    Wu et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    F3-flanked spectinomycin resistance cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIAA5::LUC

    Plasmid

    #141169

    Purpose

    Luminescent reporter for auxin signaling

    Depositor

    Vardis Ntoukakis, Patrick Schäfer

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtIAA5 promoter
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtIAA5, At1g15580

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB036d

    Plasmid

    #99216

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and PproA

    Available Since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. P303-FBP_2

    Plasmid

    #139693

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB036a

    Plasmid

    #99215

    Purpose

    Accessory plasmid for PACE of MjTyrRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK and PproD

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ND4 Right-GSVG-UGI

    Plasmid

    #187414

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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