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pcDNA3-PKCZeta KR HA Tagged
(Plasmid #24608)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24608 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKCZeta
  • Alt name
    PKC zeta
  • Species
    R. norvegicus (rat)
  • Mutation
    K281R mutation in the ATP binding domain causing a kinase dead variant.
  • GenBank ID
    NM_022507.1 NP_071952.1
  • Entrez Gene
    Prkcz (a.k.a. 14-3-3-zetaisoform, Pkcz, r14-3-3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-PKCZeta KR HA Tagged was a gift from Jeff Wrana (Addgene plasmid # 24608 ; http://n2t.net/addgene:24608 ; RRID:Addgene_24608)
  • For your References section:

    Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Wang HR, Zhang Y, Ozdamar B, Ogunjimi AA, Alexandrova E, Thomsen GH, Wrana JL. Science. 2003 Dec 5. 302(5651):1775-9. 10.1126/science.1090772 PubMed 14657501