We narrowed to 12,202 results for: 112

1. pcDNA3-FLAG -YWAA-pGK-HYG

   Plasmid

   #107381

   Purpose

   Mammalian expression of FLAG-YWAA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   cereblon (CRBN Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   CRBN-Y383A/W385A

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176538

   Purpose

   Mammalian expression of nuclear localized AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMx PylT_FLAG-Mx PylRS YA

   Plasmid

   #140012

   Purpose

   Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y126A

   Promoter

   EF1

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-Tet2 promoter F

   Plasmid

   #63879

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet2

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet2 Promoter fragment F
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MO70-N-Flag-hCRACR2A-isoform-a

   Plasmid

   #79592

   Purpose

   Transient and retroviral expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

   Insert

   CRACR2A-isoform-a (CRACR2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rosa26_gRNA

   Plasmid

   #196138

   Purpose

   gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Rosa26 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FPR3-DuET

   Plasmid

   #213241

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   FPR3 (FPR3 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH4R-mCherry-N1

   Plasmid

   #84328

   Purpose

   Histamine-4 receptor tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H4R-mCherry (HRH4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 Flag MFF iso2 WT

    Plasmid

    #74389

    Purpose

    Flag tagged human MFF isoform 2

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS1862

    Plasmid

    #205975

    Purpose

    FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5 from JAGBLH010000220, Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS1859

    Plasmid

    #205972

    Purpose

    FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS1860

    Plasmid

    #205973

    Purpose

    FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-HSF1Δ∼PRD

    Plasmid

    #71725

    Purpose

    plasmid expressing mic-his tag phosphorylation-deficient hHSF1

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    phosphorylation-deficient HSF1 (HSF1 Human)
    

    Tags

    mac 6xHIS

    Expression

    Mammalian

    Mutation

    15 phosphorylation sites in RD mutated to Alanine

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130993

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSV272-PfMBP-HypDAE

    Plasmid

    #110296

    Purpose

    Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Science. 2017 Feb 10;355(6325). pii: 355

    Insert

    t-4-Hydroxy-L-proline Dehydratase Activating Enzyme
    

    Tags

    His6 and Maltose Binding Protein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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