We narrowed to 51,385 results for: des.1

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pMK293 (mAID-mCherry2-Hygro)

Plasmid

#72831

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Hygro

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-Rac1

Plasmid

#83950

Purpose

Rac1 FLIM donor for dynamic imaging of GTPase activity

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMK292 (mAID-mCherry2-NeoR)

Plasmid

#72830

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Neo

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LAMP1

Plasmid

#110948

Purpose

Expresses LAMP1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-tmpknot-epegRNA

Plasmid

#214092

Purpose

Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-acatenin

Plasmid

#186458

Purpose

Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SypHer2-DAAO-NES

Plasmid

#119184

Purpose

Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

SypHer2-DAAO-NES

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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SGLT2(GFP)-MAP17(nb)

Plasmid

#216211

Purpose

for expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT2 GFP fusion and MAP17 nanobody fusion (PDZK1IP1, SLC5A2 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMM7

Plasmid

#201213

Purpose

Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

Depositor

Alex Nielsen

Article

Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

Inserts

sfGFP
Spo0A right flank
Spo0A left flank

Use

Synthetic Biology

Expression

Bacterial

Mutation

F11V, T13P, N39D, A179A

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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B2345_BTB2x-mCherry

Plasmid

#215097

Purpose

Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB-BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK294 (mAID-mCherry2-Bsr)

Plasmid

#72832

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Bsr

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

Plasmid

#47550

Purpose

Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Inserts

Cas9
ttTi5605 sgRNA

Use

CRISPR

Tags

HA and NLS

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Promoter

U6 and eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G12C-tmpknot-epegRNA

Plasmid

#214094

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G12C mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G12C epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G BCL2L11 Neo

Plasmid

#140747

Purpose

Inducibly expresses BIM in mammalian cells

Depositor

Scott Dixon

Article

Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

Insert

BCL2L11 (BCL2L11 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV TRE3G

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVd1-NLS-GAL4-iLight2-VP16

Plasmid

#219550

Purpose

NLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16

Use

AAV

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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