We narrowed to 18,496 results for: Met

1. pACYCDuet_M42FDHFR_R166QTS

   Plasmid

   #91236

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M42F and R166Q

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PKAcs-nolinker-GFP

   Plasmid

   #108579

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PKAcs-G12X-GFP

   Plasmid

   #108588

   Purpose

   Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

   Depositor

   Robert Hughes

   Article

   Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

   Insert

   protein kinase A, catalytic subunit
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H87Q and W196R

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBAD-MTX_Snifit

   Plasmid

   #119043

   Purpose

   Plasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)

   Depositor

   Kai Johnsson

   Article

   Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

   Insert

   MTX_Snifit
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44L H45Q

   Promoter

   pBAD

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPOR1CB0083

   Plasmid

   #117562

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Methyltransferase {AT5G10620} in A. thaliana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_Methyltransferase {AT5G10620} A. thaliana
   

   Expression

   Plant

   Promoter

   AtU6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-YJR129c

   Plasmid

   #85118

   Purpose

   bacterial expression of YJR129c

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFM3 (EFM3 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET29b-clbH-C-A2-PCP S1524A

   Plasmid

   #114158

   Purpose

   Expresses ClbH-C-A2-PCP S1524A

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Tags

   S-Tag, Thrombin site

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH with S…

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.hSyn-FLEX.iGABASnFR.F102Y.Y137L

   Plasmid

   #112165

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102Y.Y137L
   

   Use

   AAV

   Mutation

   F102Y.Y137L

   Promoter

   Synapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTalin2_R7R8

   Plasmid

   #111041

   Purpose

   Plasmid expresses the talin2 rod domains R7 and R8

   Depositor

   Ben Goult

   Article

   Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

   Insert

   TLN2 (Tln2 Mouse)
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mTalin2_R7

    Plasmid

    #111039

    Purpose

    Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

    Depositor

    Ben Goult

    Article

    Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

    Insert

    TLN2 (Tln2 Mouse)
    

    Tags

    His Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P0050::kaiBC S431A T432A NS1

    Plasmid

    #101837

    Purpose

    drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    kaiBC S431A T432A with p0050 promoter
    

    Use

    Cyanobacteria cloning vector

    Mutation

    S431A T432A

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. P0050::kaiBC S431E T432E NS1

    Plasmid

    #101836

    Purpose

    drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    kaiBC S431E T432E with p0050 promoter
    

    Use

    Cyanobacteria cloning vector

    Mutation

    S431E T432E

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp128-P30-His-pol30(K127/164R)

    Plasmid

    #99545

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUPER-retro-puro-shNup88-XbaI

    Plasmid

    #87328

    Purpose

    To express shRNA against human Nup88

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup88
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUPER-retro-puro-shNup93-XhoI

    Plasmid

    #87330

    Purpose

    To express shRNA against human Nup93

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup93
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pILGFPB6

    Plasmid

    #83557

    Purpose

    Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL10 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ARK1a

    Plasmid

    #81016

    Purpose

    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    

    Promoter

    LacUV5 and Trc promoter

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-PRMT9 deltaTPR mutant

    Plasmid

    #67614

    Purpose

    bacterial expression of GST-PRMT9 missing residues 21-139

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    truncation mutant missing aa 21-139

    Promoter

    tac

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

20. GST-PRMT9 deltaN (aa 21-350)

    Plasmid

    #67611

    Purpose

    bacterial expression of GST-PRMT9 missing residues 21-350

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    truncation mutant missing aa 21-350

    Promoter

    tac

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only